Fluorescence and sperm detection

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An independent claim is also included for a device for carrying out the above mentioned method, comprising a slide unit 1and at least one flow chamber 2which has hose gauges 3 on both sides, where the height of the flow chamber is mu m. The invention relates to a method and a device for determining the fertility of sperm. Besamung darstellt.

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First, the biological facts about semen:. Seminal fluid is a complex mixture of secretions from at least four male urogenital glands. An average male ejaculate measures around 3.

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In mammals, the success of fertilization largely depends on gamete fertility potential and consequently on what concerns sperm and oocyte quality they are both equally important. Sperm contribution to fertilization is usually estimated through evaluation of semen parameters. A loss of fertility potential associated to manipulation and preservation techniques is usually calculated based on the semen characteristics at collection and on the knowledge of the damages associated with the technique to be implemented.

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Print this page. E-mail this page. The detection of sperm and separation of sperm cells from the victim's DNA is crucial to identifying and characterizing the perpetrator's DNA profile.

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Genetic abnormalities, including sperm chromosome aneuploidy as well as structural aberrations, are one of the major causes of infertility. The use of chromosome-specific DNA probes labeled with fluorochromes, particularly the combination with multiple probes, has been used to indirectly study the sperm chromosome by fluorescent in situ hybridization FISH. Clinically, this technique is also used to assess the sperm of men recovering from gonadotoxic treatment.

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Human Genetics. Fluorescence in situ hybridization FISH was performed on human interphase sperm nuclei to determine the utility of this technique for aneuploidy detection. Repetitive DNA sequences specific for chromosomes 1, 12 and X were biotinylated and hybridized with mature sperm, which had been treated with cetyltrimethylammonium bromide and dithiothreitol to render them accessible to the probes.

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Sperm is produced by the testis and mature in the epididymis. For having a successful conception, the fertilizing sperm should have functional competent membranes, intact acrosome, functional mitochondria and an intact haploid genome. The effects of genetic and environmental factors result in sperm vulnerability to damage in the process of spermatogenesis and maturation.

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Sperm sorting is a means of choosing what type of sperm cell is to fertilize the egg cell. Several conventional techniques of centrifugation or swim-up. Newly applied methods such as flow cytometry expand the possibilities of sperm sorting and new techniques of sperm sorting are being developed.

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Forensic scientists are tasked with the collection, preservation, and analysis of scientific evidence during the course of a criminal investigation. Within the field of forensic sciences, detection of semen is the primary method to confirm sexual assault. Separation of sperm cells from the victim's DNA is crucial for identifying and characterizing the perpetrator's DNA profile.

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A variety of laboratory tests were developed to obtain more reliable results of sperm evaluation and increase the accuracy of sperm fertility predictions. These tests detected damage of sperm specific compartments or organelles, which cannot be detected in routine sperm analysis. The use of fluorescent probes and detection using fluorescent microscopy or flow cytometry is an important tool but a more precise and accurate laboratory test is needed. Propidium iodide and 6-carboxyfluorescein diacetate are used for evaluations of plasmatic membrane integrity.


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